| SPECIESREACTIVITY: | Human,Mouse,Rat |
|---|---|
| TESTEDAPPLICATIONS: | IHC,WB |
| APPLICATIONS: | WesternBlot:1:500~1:1000,Immunohistochemistry:1:50~1:100 |
| PREDICTEDMOLECULARWEIGHT: | 28kDa |
| SPECIFICITY: | Thisantibodydetectsendogenousleveloftotal14-3-3zetaprotein. |
| IMMUNOGEN: | 14-3-3zeta(Ab-58)antibodywasraisedagainstapeptidesequencearoundaa.56~60(R-S-S-W-R)derivedfromHuman14-3-3zeta. |
| HOSTSPECIES: | Rabbit |
| PURIFICATION: | Antibodieswerepurifiedbyaffinity-chromatographyusingepitope-specificpeptide. |
|---|---|
| PHYSICALSTATE: | Liquid |
| BUFFER: | Antibodysuppliedinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
| CONCENTRATION: | 1mg/mL |
| STORAGECONDITIONS: | Storeantibodyat-20˚Cforuptooneyear. |
| CLONALITY: | Polyclonal |
| CONJUGATE: | Unconjugated |
| ALTERNATENAMES: | HEL4,YWHAD,KCIP-1,HEL-S-3,14-3-3-zeta,ERBB,ERBB1,HER1,1433Z,143Z,FAS,FactoractivatingexoenzymeS |
|---|---|
| ACCESSIONNO.: | NP_001129171.1 |
| PROTEINGINO.: | 208973238 |
| OFFICIALSYMBOL: | YWHAZ |
| GENEID: | 7534 |
| BACKGROUND: | Thisgeneproductbelongstothe14-3-3familyofproteinswhichmediatesignaltransductionbybindingtophosphoserine-containingproteins.Thishighlyconservedproteinfamilyisfoundinbothplantsandmammals,andthisproteinis99%identicaltothemouse,ratandsheeporthologs.TheencodedproteininteractswithIRS1protein,suggestingaroleinregulatinginsulinsensitivity.Severaltranscriptvariantsthatdifferinthe5"UTRbutthatencodethesameproteinhavebeenidentifiedforthisgene. |
|---|---|
| REFERENCES: | 1)GuYM,etal.(2006)FEBSLett;580(1):305-310PowellDW,etal.(2003)MolCellBiol;23(15):5376-5387MackintoshC.(2004)BiochemJ;381(Pt2):329-342. |
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