| SPECIES: | Human |
|---|---|
| SOURCESPECIES: | HumanCells |
| SEQUENCE: | Glu20-Lys368 |
| FUSIONTAG: | C-6Histag |
| TESTEDAPPLICATIONS: | |
| APPLICATIONS: | ThisrecombinantproteincanbeusedforBIOLOGicalassays.Forresearchuseonly. |
| PURITY: | Greaterthan95%asdeterminedbyreducingSDS-PAGE. Endotoxinlevellessthan0.1ng/ug(1IEU/ug)asdeterminedbyLALtest. |
|---|---|
| PREDICTEDMOLECULARWEIGHT: | 40.47kD |
| PHYSICALSTATE: | Lyophilized |
| BUFFER: | Lyophilizedfroma0.2umfilteredsolutionof20mMPB,150mMNaCl,pH7.2.Itisnotrecommendedtoreconstitutetoaconcentrationlessthan100ug/ml.DissolvethelyophilizedproteininddH2O. |
| STORAGECONDITIONS: | Lyophilizedproteinshouldbestoredat-20˚C,thoughstableatroomtemperaturefor3weeks. Reconstitutedproteinsolutioncanbestoredat4-7˚Cfor2-7days. Aliquotsofreconstitutedsamplesarestableat-20˚Cfor3months. |
| ALTERNATENAMES: | Biglycan,Bone/CartilageProteoglycanI,PG-S1,BGN,SLRR1A |
|---|---|
| ACCESSIONNO.: | P21810 |
| PROTEINGINO.: | 578877726 |
| Biglycanisa200-350kDproteoglycanconsistingofa45kDcoreproteinandtwochrondroitin/dermatansulfateglycosaminoglycanchains.BiglycanbindstoTGF-ß.ItalsobindstocollagentypeIinlowionicstrength(lessthan3mMphosphate)buffer.Athigherionicstrengths,BiglycandoesnotbindtocollagentypeI.ItenhancestheinhibitioneffectofTGF-ßonosteoclastproliferationataconcentrationof4-20mg/ml.ItalsopreventstheattachmentofCHOcellstofibronectin,witha50%inhibitionat17-21mg/ml. |
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