SPECIES: | Human |
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SOURCESPECIES: | E.coli |
SEQUENCE: | Glu6-Ser294 |
FUSIONTAG: | C-6Histag |
TESTEDAPPLICATIONS: | |
APPLICATIONS: | ThisrecombinantproteincanbeusedforBIOLOGicalassays.Forresearchuseonly. |
PURITY: | Greaterthan95%asdeterminedbyreducingSDS-PAGE. Endotoxinlevellessthan0.1ng/ug(1IEU/ug)asdeterminedbyLALtest. |
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PREDICTEDMOLECULARWEIGHT: | 33.8kD |
PHYSICALSTATE: | Lyophilized |
BUFFER: | Lyophilizedfroma0.2umfilteredsolutionof4mMHCl.Itisnotrecommendedtoreconstitutetoaconcentrationlessthan100ug/ml.DissolvethelyophilizedproteininddH2O. |
STORAGECONDITIONS: | Lyophilizedproteinshouldbestoredat-20˚C,thoughstableatroomtemperaturefor3weeks. Reconstitutedproteinsolutioncanbestoredat4-7˚Cfor2-7days. Aliquotsofreconstitutedsamplesarestableat-20˚Cfor3months. |
ALTERNATENAMES: | BLVRA,BiliverdinreductaseA,BVRA,Biliverdin-IXalpha-reductase,BLVR,BVR,REF:C1004 |
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ACCESSIONNO.: | P53004 |
HumanBiliverdinreductaseA(BLVRA)isbelongedtotheGfo/Idh/MocAfamilyandBiliverdinreductasesubfamily.BLVRAisanenzymethatinhumansisencodedbytheBLVRAgene.BLVRAplaysanimportantroleinreducingthegamma-methenebridgeoftheopentetrapyrrole,biliverdinIXalpha,tobilirubinwiththeconcomitantoxidationofaNADHorNADPHcofactor.BLVRAactsonbiliverdinbyreducingitsdouble-bondbetweenthepyrroleringsintoasingle-bond.ItaccomplishesthisusingNADPH+H+asanelectrondonor,formingbilirubinandNADP+asproducts. |
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