| SPECIES: | Human |
|---|---|
| SOURCESPECIES: | E.coli |
| SEQUENCE: | Glu6-Ser294 |
| FUSIONTAG: | C-6Histag |
| TESTEDAPPLICATIONS: | |
| APPLICATIONS: | ThisrecombinantproteincanbeusedforBIOLOGicalassays.Forresearchuseonly. |
| PURITY: | Greaterthan95%asdeterminedbyreducingSDS-PAGE. Endotoxinlevellessthan0.1ng/ug(1IEU/ug)asdeterminedbyLALtest. |
|---|---|
| PREDICTEDMOLECULARWEIGHT: | 33.8kD |
| PHYSICALSTATE: | Lyophilized |
| BUFFER: | Lyophilizedfroma0.2umfilteredsolutionof4mMHCl.Itisnotrecommendedtoreconstitutetoaconcentrationlessthan100ug/ml.DissolvethelyophilizedproteininddH2O. |
| STORAGECONDITIONS: | Lyophilizedproteinshouldbestoredat-20˚C,thoughstableatroomtemperaturefor3weeks. Reconstitutedproteinsolutioncanbestoredat4-7˚Cfor2-7days. Aliquotsofreconstitutedsamplesarestableat-20˚Cfor3months. |
| ALTERNATENAMES: | BLVRA,BiliverdinreductaseA,BVRA,Biliverdin-IXalpha-reductase,BLVR,BVR,REF:C1004 |
|---|---|
| ACCESSIONNO.: | P53004 |
| HumanBiliverdinreductaseA(BLVRA)isbelongedtotheGfo/Idh/MocAfamilyandBiliverdinreductasesubfamily.BLVRAisanenzymethatinhumansisencodedbytheBLVRAgene.BLVRAplaysanimportantroleinreducingthegamma-methenebridgeoftheopentetrapyrrole,biliverdinIXalpha,tobilirubinwiththeconcomitantoxidationofaNADHorNADPHcofactor.BLVRAactsonbiliverdinbyreducingitsdouble-bondbetweenthepyrroleringsintoasingle-bond.ItaccomplishesthisusingNADPH+H+asanelectrondonor,formingbilirubinandNADP+asproducts. |
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