SPECIES: | Human |
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SOURCESPECIES: | E.coli |
SEQUENCE: | PGAEGPRGFPGIQGRKGEPGEGAYVYRSAFSVGLETYVTIPNMPIRFTKIFYNQQNHYDGSTGKFHCNIPGLYYFAYHITVYMKDVKVSLFKKDKAMLFTYDQYQENNVDQASGSVLLHLEVGDQVWLQVYGEGERNGLYADNDNDSTFTGFLLYHDTN |
TESTEDAPPLICATIONS: | |
BIOLOGICALACTIVITY: | BiologicalactivitywasdeterminedbyacytotoxicityassayusingM1cells.TheexpectedED50forthiseffectis0.5-1.0μg/mL. |
PURITY: | Greaterthan98%bySDS-PAGEgelandHPLCanalyses. Endotoxinlevelislessthan0.1ngperμg(1EU/μg). |
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PHYSICALSTATE: | Lyophilized |
STORAGECONDITIONS: | ThelyophilizedAcrp30recombinantproteinisstableforatleast2yearsfromdateofreceiptat-20˚C.ReconstitutedAcrp30isstableforatleast3monthswhenstoredinworkingaliquotswithacarrierproteinat-20˚C.Aswithanyprotein,exposingAcrp30recombinantproteintorepeatedfreeze/thawcyclesisnotrecommended.Whenworkingwithproteinscareshouldbetakentokeeprecombinantproteinatacoolandstabletemperature. |
ALTERNATENAMES: | ACDC,ADPN,APM1,APM-1,GBP28,ACRP30,ADIPQTL1,ACDC,Adiponectin,30kDaadipocytecomplement-relatedprotein |
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ACCESSIONNO.: | NP_001171271.1 |
PROTEINGINO.: | 295317372 |
ThegAcrp30variantisanaturallyoccurringglobularprotein,obtainedbyproteolyticprocessingofadiponectin.Adiponectinisproducedandsecretedexclusivelybyadipocytes,andisarelativelyabundantplasmaprotein,accountingforupto0.05%oftotalserumprotein.LikeAdiponectin,Acrp30iscapableofdecreasinghyperglycemiaandreversinginsulinresistance.Additionally,gAcrp30hasbeenshowntobeanimportantfactorinpromotingfatlossbysignalingmuscletoabsorbandburnFree-FattyAcids(FFAs).ThesignalingreceptorsforadiponectinandgAcrp30haverecentlybeenidentifiedandnamesAdipoR1andAdipoR2.AdipoR2ispredominantlyexpressedintheliver.ThisnaturallyoccurringvariantofhumangAcrp30/Adipoleanisan18.1kDaprotein,containing14aminoacidsextraattheN-terminusofhumangAcrp30/Adipolean. |
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