SPECIESREACTIVITY: | Human |
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TESTEDAPPLICATIONS: | ELISA,WB |
APPLICATIONS: | A4GNTantibodycanbeusedfordetectionofA4GNTbyELISAat1:62500.A4GNTantibodycanbeusedfordetectionofA4GNTbywesternblotat0.25μg/mL,andHRPconjugatedsecondaryantibodyshouldbediluted1:50,000-100,000. |
USERNOTE: | Optimaldilutionsforeachapplicationtobedeterminedbytheresearcher. |
POSITIVECONTROL: | 1)Cat.No.1211-HepG2CellLysate |
PREDICTEDMOLECULARWEIGHT: | 39kDa |
IMMUNOGEN: | AntibodyproducedinrabbitsimmunizedwithasyntheticpeptidecorrespondingaregionofhumanA4GNT. |
HOSTSPECIES: | Rabbit |
PURIFICATION: | Antibodyispurifiedbypeptideaffinitychromatographymethod. |
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PHYSICALSTATE: | Lyophilized |
BUFFER: | AntibodyislyophilizedinPBSbufferwith2%sucrose.Add50μLofdistilledwater.Finalantibodyconcentrationis1mg/mL. |
CONCENTRATION: | 1mg/ml |
STORAGECONDITIONS: | Forshortperiodsofstorage(days)storeat4˚C.Forlongerperiodsofstorage,storeA4GNTantibodyat-20˚C.Aswithanyantibodyavoidrepeatfreeze-thawcycles. |
CLONALITY: | Polyclonal |
CONJUGATE: | Unconjugated |
ALTERNATENAMES: | A4GNT,MGC149493,alpha4GnT |
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ACCESSIONNO.: | NP_057245 |
PROTEINGINO.: | 7705859 |
OFFICIALSYMBOL: | A4GNT |
GENEID: | 51146 |
BACKGROUND: | A4GNTisaproteinfromtheglycosyltransferase32family.TheenzymecatalyzesthetransferofN-acetylglucosamine(GlcNAc)tocore2branchedO-glycans.Itformsauniqueglycan,GlcNAcalpha1-->4Galbeta-->RandislargelyassociatedwiththeGolgiapparatusmembrane.Thisgeneencodesaproteinfromtheglycosyltransferase32family.TheenzymecatalyzesthetransferofN-acetylglucosamine(GlcNAc)tocore2branchedO-glycans.Itformsauniqueglycan,GlcNAcalpha1-->4Galbeta-->RandislargelyassociatedwiththeGolgiapparatusmembrane.SequenceNote:TheRefSeqtranscriptandproteinwerederivedfromgenomicsequencetomakethesequenceconsistentwiththereferencegenomeassembly.Thegenomiccoordinatesusedforthetranscriptrecordwerebasedonalignments. |
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REFERENCES: | 1)Ishizone,S.,(2006)CancerSci.97(2),119-126. |
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