SPECIES: | Human |
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SOURCESPECIES: | High-5Insectcells |
SEQUENCE: | RGHHHHHHHHETTTQGPGVLLPLPKGACTGWMAGIPGHPGHNGAPGRDGRDGTPGEKGEKGDPGLIGPKGDIGETGVPGAEGPRGFPGIQGRKGEPGEGAYVYRSAFSVGLETYVTIPNMPIRFTKIFYNQQNHYDGSTGKFYCNIPGLYYFSYHITVYMKDVKVSLFKKDKAVLFTYDQYQEKNVDQASGSVLLHLEVGDQVWLQVYGDGDHNGLYADNVNDSTFTGFLLYHDTN |
TESTEDAPPLICATIONS: | |
BIOLOGICALACTIVITY: | BiologicalactivitywasdeterminedbyacytotoxicassayusingM1cells.TheED50forthiseffectis3.0-6.0μg/mL. |
PURITY: | Greaterthan97%bySDS-PAGEgelandHPLCanalyses. Endotoxinlevelislessthan0.1ngperμg(1EU/μg). |
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PHYSICALSTATE: | Lyophilized |
STORAGECONDITIONS: | ThelyophilizedADIponectinrecombinantproteinisstableforatleast2yearsfromdateofreceiptat-20˚C.ReconstitutedAdiponectinisstableforatleast3monthswhenstoredinworkingaliquotswithacarrierproteinat-20˚C.Aswithanyprotein,exposingAdiponectinrecombinantproteintorepeatedfreeze/thawcyclesisnotrecommended.Whenworkingwithproteinscareshouldbetakentokeeprecombinantproteinatacoolandstabletemperature. |
ALTERNATENAMES: | ACDC,ADPN,APM1,APM-1,GBP28,ACRP30,ADIPQTL1,ACDC,Adiponectin,30kDaadipocytecomplement-relatedprotein |
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ACCESSIONNO.: | NP_001171271.1 |
PROTEINGINO.: | 295317372 |
Adiponectinisanadipose-derivedsecretedproteincontaining226aminoacidresidues.Itisrelativelyabundantinhumansandrodents,accountingforabout0.01%oftotalplasmaprotein.Thecirculatinglevelsofadiponectinaredecreasedunderconditionsofobesity,insulinresistance,andtypeIIdiabetes.Disruptionofadiponectininmicecausesinsulinresistanceandneointimalformation.Conversely,admiNISTrationofrecombinantadiponectinsuppresseshepaticglucoseproduction,andreversesinsulinresistanceassociatedwithbothlipoatrophyandobesity.Theprotectiveroleofadiponectinisattributedtoitsanti-inflammatoryproperties(e.g.ABIlitytosuppressexpressionofTNF-αandclassAscavengerreceptorinmacrophages).RecombinantadiponectinisamultimericglycoproteincontainingaminoacidsGlu-19toAsn-244oftheadiponectinprecursorproteinfusedtoanN-terminalhistidinetag.Monomericglycosylatedadiponectinmigratesatanapparentmolecularweightofapproximately25.9kDabySDSPAGEanalysisunderreducingconditions. |
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