LOCATION: | ACTN1peptidecorrespondsto14aminoacidsofACTN1. |
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SPECIES: | Human,Mouse,Rat |
TESTEDAPPLICATIONS: | Blocking |
APPLICATIONS: | ACTN1peptideisusedforblockingtheactivityofACTN1antibody(catalognumber43-554).TheACTN1antibodyshipsat0.5mg/mLandtheSTK36Peptideshipsasa100ugpellet.Whenpeptideisreconstitutedin200uLwater,theconcentrationwillbe0.5mg/mL,identicaltothatoftheantibody.Thebeststartingratiowouldbe1:1(whichmeansmolarexcessofpeptiderelativetoantibodywhenidenticalvolumesaremixed).Mixequalvolumeofpeptideandantibodyattherequireddilutionandleaveatambienttemperaturefor20minutes.Thenincubateanddevelopinparalleltwoblots:onewiththeantibodyandonewiththeantibodywhichhasbeenpre-absorbedwiththeprovidedpeptide.. |
PHYSICALSTATE: | Lyophilized |
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BUFFER: | ACTN1peptideissuppliedas100μgofdriedpeptide.Whenthepeptideisreconstitutedin200μLwater,theconcentrationwillbe0.5mg/mL. |
STORAGECONDITIONS: | Storeat-20˚C.Whenreconstitutedminimizefreezingandthawing. |
ALTERNATENAMES: | ACTN1,actinin,alpha1,F-actincross-linkingprotein,actinin1smoothmuscle,alpha-actinincytoskeletalisoform,alpha-actinin-1,non-musclealpha-actinin-1 |
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ACCESSIONNO.: | NP_001123476.1,NP_001093.1,NP_001123477.1 |
PROTEINGINO.: | 4501891 |
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