SPECIES: | Human |
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SOURCESPECIES: | E.coli |
SEQUENCE: | Gly2-Pro505 |
FUSIONTAG: | C-6Histag |
TESTEDAPPLICATIONS: | |
APPLICATIONS: | ThisrecombinantproteincanbeusedforBIOLOGicalassays.Forresearchuseonly. |
PURITY: | Greaterthan95%asdeterminedbyreducingSDS-PAGE. Endotoxinlevellessthan0.1ng/ug(1IEU/ug)asdeterminedbyLALtest. |
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PREDICTEDMOLECULARWEIGHT: | 58.7kD |
PHYSICALSTATE: | Liquid |
BUFFER: | Suppliedasa0.2umfilteredsolutionof20mMTris,500mMNaCl,1mMDTT,pH7.4.Itisnotrecommendedtoreconstitutetoaconcentrationlessthan100ug/ml.DissolvethelyophilizedproteininddH2O. |
STORAGECONDITIONS: | Storeat-20˚C,stablefor6monthsafterreceipt. Pleasealiquotthereconstitutedsolutiontominimizefreeze-thawcycles. |
ALTERNATENAMES: | Tyrosine-ProteinKinaseBlk,BLymphocyteKinase,p55-Blk,BLK |
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ACCESSIONNO.: | P51451 |
Tyrosine-ProteinKinaseBlk(BLK)containsoneproteinkinasedomain,oneSH2domainandoneSH3domain.BLKisanon-receptortyrosinekinase,whichisinvolvedinB-lymphocytedevelopment,differentiationandsignaling.B-cellreceptor(BCR)signalingrequiresatightregulationofseveralproteintyrosinekinasesandphosphatases,andassociatedcoreceptors.SignalingthroughBLKplaysanimportantroleintransmittingsignalsthroughsurfaceimmunoglobulinesandsupportsthepro-Btopre-Btransition,aswellasthesignalingforgrowtharrestandapoptosisdownstreamofB-cellreceptor.DefectsinBLKareacauseofmaturity-onsetdiabetesoftheyoungtype11(MODY11). |
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