| SPECIES: | Human |
|---|---|
| SOURCESPECIES: | E.coli |
| SEQUENCE: | Gly2-Pro505 |
| FUSIONTAG: | C-6Histag |
| TESTEDAPPLICATIONS: | |
| APPLICATIONS: | ThisrecombinantproteincanbeusedforBIOLOGicalassays.Forresearchuseonly. |
| PURITY: | Greaterthan95%asdeterminedbyreducingSDS-PAGE. Endotoxinlevellessthan0.1ng/ug(1IEU/ug)asdeterminedbyLALtest. |
|---|---|
| PREDICTEDMOLECULARWEIGHT: | 58.7kD |
| PHYSICALSTATE: | Liquid |
| BUFFER: | Suppliedasa0.2umfilteredsolutionof20mMTris,500mMNaCl,1mMDTT,pH7.4.Itisnotrecommendedtoreconstitutetoaconcentrationlessthan100ug/ml.DissolvethelyophilizedproteininddH2O. |
| STORAGECONDITIONS: | Storeat-20˚C,stablefor6monthsafterreceipt. Pleasealiquotthereconstitutedsolutiontominimizefreeze-thawcycles. |
| ALTERNATENAMES: | Tyrosine-ProteinKinaseBlk,BLymphocyteKinase,p55-Blk,BLK |
|---|---|
| ACCESSIONNO.: | P51451 |
| Tyrosine-ProteinKinaseBlk(BLK)containsoneproteinkinasedomain,oneSH2domainandoneSH3domain.BLKisanon-receptortyrosinekinase,whichisinvolvedinB-lymphocytedevelopment,differentiationandsignaling.B-cellreceptor(BCR)signalingrequiresatightregulationofseveralproteintyrosinekinasesandphosphatases,andassociatedcoreceptors.SignalingthroughBLKplaysanimportantroleintransmittingsignalsthroughsurfaceimmunoglobulinesandsupportsthepro-Btopre-Btransition,aswellasthesignalingforgrowtharrestandapoptosisdownstreamofB-cellreceptor.DefectsinBLKareacauseofmaturity-onsetdiabetesoftheyoungtype11(MODY11). |
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