| SPECIES: | Mouse |
|---|---|
| SOURCESPECIES: | HumanCells |
| SEQUENCE: | Val38-Lys245 |
| FUSIONTAG: | C-Fctag |
| TESTEDAPPLICATIONS: | |
| APPLICATIONS: | ThisrecombinantproteincanbeusedforBIOLOGicalassays.Forresearchuseonly. |
| PURITY: | Greaterthan95%asdeterminedbyreducingSDS-PAGE. Endotoxinlevellessthan0.1ng/ug(1IEU/ug)asdeterminedbyLALtest. |
|---|---|
| PREDICTEDMOLECULARWEIGHT: | 50.8kD |
| PHYSICALSTATE: | Lyophilized |
| BUFFER: | Lyophilizedfroma0.2umfilteredsolutionofPBS,pH7.4.Itisnotrecommendedtoreconstitutetoaconcentrationlessthan100ug/ml.DissolvethelyophilizedproteininddH2O. |
| STORAGECONDITIONS: | Lyophilizedproteinshouldbestoredat-20˚C,thoughstableatroomtemperaturefor3weeks. Reconstitutedproteinsolutioncanbestoredat4-7˚Cfor2-7days. Aliquotsofreconstitutedsamplesarestableat-20˚Cfor3months. |
| ALTERNATENAMES: | T-lymphocyteactivationantigenCD80,ActivationB7-1antigen,B7,CD80 |
|---|---|
| ACCESSIONNO.: | Q00609 |
| ClusterofDifferentiation80,alsocalledB7-1,isamemberofcellsurfaceimmunoglobulinsuperfamilywhichplayskey,yetdistinctrolesintheactivationofTcells.ItistheligandfortwodifferentproteinsontheTcellsurface:CD28andCTLA-4.StudieshaveshownthatCTLA-4bindsmostlytoCD80.Thestructurepresentstwoextracellulardomains:amembranedistalvariable-likedomain(IgV)andamembraneproximalIgconstant-likedomain(IgC)alongwithanintracellulardomain.BothIgVandIgCconsistofanti-parallelbetasandwichesjoinedbyashortlinkerregion.CD80ismostlyexpressedonthesurfaceofantigen-presentingcellsincludingactivatedBcells,macrophagesanddendriticcells. |
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