SPECIES REACTIVITY: | Human, Mouse |
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TESTED APPLICATIONS: | Flow, WB |
APPLICATIONS: | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 |
USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
PREDICTED MOLECULAR WEIGHT: | 32 kDa |
IMMUNOGEN: | This ACER3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 224-250 amino acids from the C-terminal region of human ACER3. |
HOST SPECIES: | Rabbit |
PURIFICATION: | This antibody is purified through a protein A column, followed by peptide affinity purification. |
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PHYSICAL STATE: | Liquid |
BUFFER: | Supplied in PBS with 0.09% (W/V) sodium azide. |
CONCENTRATION: | 0.48 mg/ml |
STORAGE CONDITIONS: | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
CLONALITY: | Polyclonal |
ISOTYPE: | Rabbit Ig |
CONJUGATE: | Unconjugated |
ALTERNATE NAMES: | Alkaline ceramidase 3, AlkCDase 3, Alkaline CDase 3, 351-, Alkaline dihydroceramidase SB89, Alkaline phytoceramidase, aPHC, ACER3, APHC, PHCA |
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ACCESSION NO.: | Q9NUN7 |
PROTEIN GI NO.: | 296439452 |
OFFICIAL SYMBOL: | ACER3 |
GENE ID: | 55331 |
BACKGROUND: | ACER3 hydrolyzes only phytoceramide into phytosphingosine and free fatty acid. Does not have reverse activity. |
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REFERENCES: | 1) Wheeler,H.E., et.al., PLoS Genet.(10), E1000685 (2009) | 2) Mao,C. et.al., Biochim. Biophys. Acta 1781 (9), 424-434 (2008) |
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