| SPECIES REACTIVITY: | Human, Mouse |
|---|---|
| TESTED APPLICATIONS: | Flow, WB |
| APPLICATIONS: | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
| PREDICTED MOLECULAR WEIGHT: | 32 kDa |
| IMMUNOGEN: | This ACER3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 224-250 amino acids from the C-terminal region of human ACER3. |
| HOST SPECIES: | Rabbit |
| PURIFICATION: | This antibody is purified through a protein A column, followed by peptide affinity purification. |
|---|---|
| PHYSICAL STATE: | Liquid |
| BUFFER: | Supplied in PBS with 0.09% (W/V) sodium azide. |
| CONCENTRATION: | 0.48 mg/ml |
| STORAGE CONDITIONS: | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| CLONALITY: | Polyclonal |
| ISOTYPE: | Rabbit Ig |
| CONJUGATE: | Unconjugated |
| ALTERNATE NAMES: | Alkaline ceramidase 3, AlkCDase 3, Alkaline CDase 3, 351-, Alkaline dihydroceramidase SB89, Alkaline phytoceramidase, aPHC, ACER3, APHC, PHCA |
|---|---|
| ACCESSION NO.: | Q9NUN7 |
| PROTEIN GI NO.: | 296439452 |
| OFFICIAL SYMBOL: | ACER3 |
| GENE ID: | 55331 |
| BACKGROUND: | ACER3 hydrolyzes only phytoceramide into phytosphingosine and free fatty acid. Does not have reverse activity. |
|---|---|
| REFERENCES: | 1) Wheeler,H.E., et.al., PLoS Genet.(10), E1000685 (2009) | 2) Mao,C. et.al., Biochim. Biophys. Acta 1781 (9), 424-434 (2008) |
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