SPECIESREACTIVITY: | C.elegans,Human,Mouse,Rat |
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TESTEDAPPLICATIONS: | ELISA,WB |
APPLICATIONS: | ACO2antibodycanbeusedfordetectionofACO2byELISAat1:312500.ACO2antibodycanbeusedfordetectionofACO2bywesternblotat1μg/mL,andHRPconjugatedsecondaryantibodyshouldbediluted1:50,000-100,000. |
USERNOTE: | Optimaldilutionsforeachapplicationtobedeterminedbytheresearcher. |
POSITIVECONTROL: | 1)721_BCellLysate |
PREDICTEDMOLECULARWEIGHT: | 82kDa |
IMMUNOGEN: | AntibodyproducedinrabbitsimmunizedwithasyntheticpeptidecorrespondingaregionofhumanACO2. |
HOSTSPECIES: | Rabbit |
PURIFICATION: | Antibodyispurifiedbypeptideaffinitychromatographymethod. |
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PHYSICALSTATE: | Lyophilized |
BUFFER: | AntibodyislyophilizedinPBSbufferwith2%sucrose.Add50μLofdistilledwater.Finalantibodyconcentrationis1mg/mL. |
CONCENTRATION: | 1mg/ml |
STORAGECONDITIONS: | Forshortperiodsofstorage(days)storeat4˚C.Forlongerperiodsofstorage,storeACO2antibodyat-20˚C.Aswithanyantibodyavoidrepeatfreeze-thawcycles. |
CLONALITY: | Polyclonal |
CONJUGATE: | Unconjugated |
ALTERNATENAMES: | ACO2,ACONM,MGC20605,MGC33908,ICRD |
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ACCESSIONNO.: | NP_001089 |
PROTEINGINO.: | 4501867 |
OFFICIALSYMBOL: | ACO2 |
GENEID: | 50 |
BACKGROUND: | ACO2belongstotheaconitase/IPMisomerasefamily.Itisanenzymethatcatalyzestheinterconversionofcitratetoisocitrateviacis-aconitateinthesecondstepoftheTCAcycle.Thisproteinisencodedinthenucleusandfunctionsinthemitochondrion.Itwasfoundtobeoneofthemitochondrialmatrixproteinsthatarepreferentiallydegradedbytheserineprotease15(PRSS15),alsoknownasLonprotease,afteroxidativemodification.Theproteinencodedbythisgenebelongstotheaconitase/IPMisomerasefamily.Itisanenzymethatcatalyzestheinterconversionofcitratetoisocitrateviacis-aconitateinthesecondstepoftheTCAcycle.Thisproteinisencodedinthenucleusandfunctionsinthemitochondrion.Itwasfoundtobeoneofthemitochondrialmatrixproteinsthatarepreferentiallydegradedbytheserineprotease15(PRSS15),alsoknownasLonprotease,afteroxidativemodification.PublicationNote:ThisRefSeqrecordincludesasubsetofthepublicationsthatareavailableforthisgene.PleaseseetheEntrezGenerecordtoaccessadditionalpublications. |
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REFERENCES: | 1)Yu,Z.,(2006)Prostate66(10),1061-1069. |
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