LOCATION: | ALOX5APpeptidecorrespondsto14aminoacidsneartheC-terminusregionofALOX5AP. |
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SPECIES: | Human |
TESTEDAPPLICATIONS: | Blocking |
APPLICATIONS: | ALOX5APPeptideisusedforblockingtheactivityofALOX5APantibody(catalognumber45-241).TheALOX5APantibodyshipsat0.5mg/mLandtheALOX5APPeptideshipsasa100ugpellet.Whenpeptideisreconstitutedin200uLwater,theconcentrationwillbe0.5mg/mL,identicaltothatoftheantibody.Thebeststartingratiowouldbe1:1(whichmeansmolarexcessofpeptiderelativetoantibodywhenidenticalvolumesaremixed).Mixequalvolumeofpeptideandantibodyattherequireddilutionandleaveatambienttemperaturefor20minutes.Thenincubateanddevelopinparalleltwoblots:onewiththeantibodyandonewiththeantibodywhichhasbeenpre-absorbedwiththeprovidedpeptide. |
PHYSICALSTATE: | Lyophilized |
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BUFFER: | ALOX5APpeptideissuppliedas100μgofdriedpeptide.Whenthepeptideisreconstitutedin200μLwater,theconcentrationwillbe0.5mg/mL. |
STORAGECONDITIONS: | Storeat-20˚C.Whenreconstitutedminimizefreezingandthawing. |
ALTERNATENAMES: | FLAP,FLAP,Arachidonate5-lipoxygenase-activatingprotein, |
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ACCESSIONNO.: | NP_001620 |
PROTEINGINO.: | 15718675 |
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