| LOCATION: | ALOX5APpeptidecorrespondsto13aminoacidsneartheintermediatedomainofALOX5AP. |
|---|---|
| SPECIES: | Human |
| TESTEDAPPLICATIONS: | Blocking |
| APPLICATIONS: | ALOX5APPeptideisusedforblockingtheactivityofALOX5APantibody(catalognumber45-242).TheALOX5APantibodyshipsat0.5mg/mLandtheALOX5APPeptideshipsasa100ugpellet.Whenpeptideisreconstitutedin200uLwater,theconcentrationwillbe0.5mg/mL,identicaltothatoftheantibody.Thebeststartingratiowouldbe1:1(whichmeansmolarexcessofpeptiderelativetoantibodywhenidenticalvolumesaremixed).Mixequalvolumeofpeptideandantibodyattherequireddilutionandleaveatambienttemperaturefor20minutes.Thenincubateanddevelopinparalleltwoblots:onewiththeantibodyandonewiththeantibodywhichhasbeenpre-absorbedwiththeprovidedpeptide. |
| PHYSICALSTATE: | Lyophilized |
|---|---|
| BUFFER: | ALOX5APpeptideissuppliedas100μgofdriedpeptide.Whenthepeptideisreconstitutedin200μLwater,theconcentrationwillbe0.5mg/mL. |
| STORAGECONDITIONS: | Storeat-20˚C.Whenreconstitutedminimizefreezingandthawing. |
| ALTERNATENAMES: | FLAP,FLAP,Arachidonate5-lipoxygenase-activatingprotein, |
|---|---|
| ACCESSIONNO.: | NP_001620.2 |
| PROTEINGINO.: | 15718675 |
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